new strategies combining sensitive pathogenic bacterial detection and high antimicrobial efficacy are urgently desirable. here, we report smart triple-functional au–ag-stuffed nanopancakes (aas-nps) exhibiting (1) controllably oxidative ag-etching thickness for simultaneously obtaining the best surface-enhanced raman scattering (sers) enhancement and high ag-loading antibacterial drug delivery, (2) expressive ag -accelerated releasing capability under neutral phosphate-buffered saline (pbs) (ph ∼ 7.4) stimulus and robust antibacterial effectiveness involving sustainable ag release, and (3) three-in-one features combining specific discrimination, sensitive detection, and inactivation of different pathogenic bacteria. originally, aas-nps were synthesized by particle growth of the selective ag-etched au@ag nanoparticles with k3[fe(cn)6], followed by the formation of an unstable prussian blue analogue for specifically binding with bacteria through the cyano group. using specific bacterial “fingerprints” resulting from the introduction of dual-function 4-mercaptophenylboronic acid (4-mpba, serving as both the sers tag and internal standard) and a sers sandwich nanostructure that was made of bacteria/sers tags/aas-nps, three bacteria (e. coli, s. aureus, and p. aeruginosa) were highly sensitively discriminated and detected, with a limit of detection of 7 cfu ml–1. meanwhile, aas-nps killed 99% of 1 × 105 cfu ml–1 bacteria within 60 min under pbs (ph ∼ 7.4) pretreatment.
